Difference between revisions of "Part:BBa K218018"

Latest revision as of 02:20, 22 October 2009

LuxO D47E with strong RBS

This is an intermediate part of an incomplete circuit that is supposed to contain B0034-K218015-B0015-K131017. LuxOD47E is one of only a few proteins that acts directly on a promoter: the qrr4 promoter. Because LuxOD47E (K218015) mimics the phosphorylated, active form of the LuxO protein (Vibrio harveyi ), it is, when expressed, able to bind to the qrr4 promoter (K131017) and activate transcription of any gene placed downstream of this promoter. Any promoter could therefore be placed in front of this circuit and any gene could be placed downstream, and it would be transcribed.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 136
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 824
Illegal SapI.rc site found at 786

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 __NOTOC__
 <partinfo>BBa_K218018 short</partinfo>
-Blah
+This is an intermediate part of an incomplete circuit that is supposed to contain B0034-K218015-B0015-K131017.   LuxOD47E is one of only a few proteins that acts directly on a promoter: the qrr4 promoter.  Because LuxOD47E (K218015) mimics the phosphorylated, active form of the LuxO protein (''Vibrio harveyi'' ), it is, when expressed, able to bind to the qrr4 promoter (K131017) and activate transcription of any gene placed downstream of this promoter.   Any promoter could therefore be placed in front of this circuit and any gene could be placed downstream, and it would be transcribed.
 <!-- Add more about the biology of this part here