Difference between revisions of "Part:BBa K5195010"
| Line 4: | Line 4: | ||
[[File:hek transfection.png|framesless|50px]] | [[File:hek transfection.png|framesless|50px]] | ||
| + | |||
| + | https://static.igem.wiki/teams/5195/hek-transfection.png | ||
Revision as of 08:11, 1 October 2024
Usage and Biology: In order to be able to quantify how much DUX4-DBD protein would be produced in cells upon transfection, we constructed a coding sequence consisting of an mNeonGreen (mNG) sequence followed by a Peptide 2A (P2A) linker upstream of the DUX4-DBD. The P2A linker causes ribosomal “skipping” during translation, and this effectively separates the mNG and DUX4-DBD proteins. In designing the coding sequence in this way, we enabled quantification of DUX4-DBD without directly attaching another large protein to it.
