Difference between revisions of "Part:BBa K5301019"
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This pathway expresses MSP1E3D1 using T7 expression system. | This pathway expresses MSP1E3D1 using T7 expression system. | ||
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Revision as of 07:58, 1 October 2024
MSP1E3D1 expression pathway (T7)
This pathway expresses MSP1E3D1 using T7 expression system.
Description
We used this pathway to express MSP1E3D1 by using T7 expression system in E.coli, and the expression of MSP1E3D1 can be induced by IPTG. For more information on MSP1E3D1, go to BBa_K5301000.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 173
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 173
Illegal NheI site found at 903 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 173
Illegal BglII site found at 799
Illegal XhoI site found at 695 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 173
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 173
- 1000COMPATIBLE WITH RFC[1000]