Difference between revisions of "Part:BBa K5291038:Design"

 
(Design Notes)
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===Design Notes===
 
===Design Notes===
Note use in strains with t7 polymerase
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The T<sub>7</sub> promoter is a strong, synthetic bacterial promoter used in molecular cloning and protein expression. It is recognized by the T<sub>7</sub> RNA polymerase, leading to high levels of gene transcription and protein production in host cells containing the T<sub>7</sub> polymerase gene.
 
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===Source===
 
===Source===

Revision as of 07:36, 1 October 2024


T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The T7 promoter is a strong, synthetic bacterial promoter used in molecular cloning and protein expression. It is recognized by the T7 RNA polymerase, leading to high levels of gene transcription and protein production in host cells containing the T7 polymerase gene.

Source

The T7 promoter originates from the T7 bacteriophage and is widely used in molecular biology for driving the transcription of genes into mRNA

References