Difference between revisions of "Part:BBa K239000:Experience"
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| + | The DegP promoter is dependent of Sigma E (and hence also the growth phase) and can not be induced only by over expression of e.g. CpxR. In comparison, the related Spy promoter (BBa_K239001), which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009) Experiments by the The UCL_London_2009 team have also showed that the activity of the DegP promoter, compared to the spy promoter, is much more dependent of the growth phase of the bacteria. | ||
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| + | For detailed information please visit | ||
| + | [http://2009.igem.org/Team:UCL_London/From_the_lab/Expts UCL_London Wet Lab Experiments] | ||
| + | &[http://2009.igem.org/Team:UCL_London/From_the_lab/Results UCL_London Wet Lab Results] | ||
Revision as of 02:07, 22 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K239000
User Reviews
UNIQc8f3d7bb7b0e4040-partinfo-00000000-QINU UNIQc8f3d7bb7b0e4040-partinfo-00000001-QINU
The DegP promoter is dependent of Sigma E (and hence also the growth phase) and can not be induced only by over expression of e.g. CpxR. In comparison, the related Spy promoter (BBa_K239001), which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009) Experiments by the The UCL_London_2009 team have also showed that the activity of the DegP promoter, compared to the spy promoter, is much more dependent of the growth phase of the bacteria.
For detailed information please visit [http://2009.igem.org/Team:UCL_London/From_the_lab/Expts UCL_London Wet Lab Experiments] &[http://2009.igem.org/Team:UCL_London/From_the_lab/Results UCL_London Wet Lab Results]