Difference between revisions of "Part:BBa K5291029:Design"

 
(→‎Source)
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Pseudomonas putida NBRC 14164
+
<i>Pseudomonas putida</i> NBRC 14164
  
 
===References===
 
===References===

Revision as of 07:09, 1 October 2024


pBBRMCS2-bcsA-bcsB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2282
    Illegal PstI site found at 3431
    Illegal PstI site found at 3464
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2282
    Illegal PstI site found at 3431
    Illegal PstI site found at 3464
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2282
    Illegal BglII site found at 1527
    Illegal BamHI site found at 1204
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2282
    Illegal PstI site found at 3431
    Illegal PstI site found at 3464
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2282
    Illegal PstI site found at 3431
    Illegal PstI site found at 3464
    Illegal NgoMIV site found at 265
    Illegal NgoMIV site found at 802
    Illegal NgoMIV site found at 814
    Illegal NgoMIV site found at 1040
    Illegal NgoMIV site found at 3355
    Illegal NgoMIV site found at 3985
    Illegal AgeI site found at 1994
    Illegal AgeI site found at 4065
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design considerations for the BcsB genes in bacterial cellulose biosynthesis involve their essential roles in forming the cellulose synthase complex, ensuring proper membrane integration, and facilitating efficient cellulose chain synthesis and export.


Source

Pseudomonas putida NBRC 14164

References