Difference between revisions of "Part:BBa K194003"
 
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K194003 short</partinfo>
 
<partinfo>BBa_K194003 short</partinfo>
−
 
+
This biobrick will allow insertion of PCR fragments using the novel USERTM biobrick assembly standard (BBF RFC 39). It contains a PacI/Nt.BbvCI USER cassette for insertion of PCR fragments using the USER cloning standard. PCR fragments containing uracil is treated with a uracil DNA glycosylase that removes the uracil exposing a predesigned 8bp overhang allowing for cloning without the need for ligase. For more information concerning USER cloning see our wiki: http://2009.igem.org/Team:DTU_Denmark/USERprinciple
−
This biobrick contains a PacI/Nt.BbvCI USER cassette for insertion of PCR fragments using the USER cloning standard (see http://2009.igem.org/Team:DTU_Denmark/project). PCR fragments containing uracil is treated with a uracil DNA glycosylase that removes the uracil exposing a predesigned 8bp overhang allowing for cloning without the need for ligase.
+
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:01, 22 October 2009

USER cassette for insertion of USER fragments This biobrick will allow insertion of PCR fragments using the novel USERTM biobrick assembly standard (BBF RFC 39). It contains a PacI/Nt.BbvCI USER cassette for insertion of PCR fragments using the USER cloning standard. PCR fragments containing uracil is treated with a uracil DNA glycosylase that removes the uracil exposing a predesigned 8bp overhang allowing for cloning without the need for ligase. For more information concerning USER cloning see our wiki: http://2009.igem.org/Team:DTU_Denmark/USERprinciple

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]