Difference between revisions of "Part:BBa K5301014"

 
Line 3: Line 3:
 
<partinfo>BBa_K5301014 short</partinfo>
 
<partinfo>BBa_K5301014 short</partinfo>
  
−
sGFP11 is an engineered version of the final strand of the GFP beta-barrel. The isolated GFP1-10 fragment does not fluoresce on its own but emits fluorescence after self-assembling with GFP11 to form GFP. GFP11 can self-assemble with GFP1-10 to form GFP, thereby emitting fluorescence. We use the self-assembly of GFP1-10 and GFP11 to construct membrane protein dimers.
 
  
 +
sGFP11 is the smaller fragment of the Split-GFP system, encompassing the final β-strand domain of green fluorescent protein (GFP).The isolated GFP11 fragment does not fluoresce on its own and is typically fused to the C-terminus of the target protein. Upon binding with the GFP1-10 fragment, it facilitates the restoration of GFP's fluorescent activity.
  
 +
 +
===Usage and Biology===
 +
 +
In our team's experiments, sGFP1-10 and sGFP11 are individually fused and expressed with the target proteins.We use the self-assembly of GFP1-10 and GFP11 to construct membrane protein dimers.
 +
 +
 +
===Characterization===
 +
 +
We performed an in vitro mixing and incubation of sGFP1-10 with sGFP11 to achieve their binding. The images captured under a fluorescence microscope are shown in the figure below.
 +
 +
 +
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:min-content;"><div style="zoom:0.25;overflow:hidden;">
 +
https://static.igem.wiki/teams/5301/parts/sgfp-fluorescent.png
 +
</div><div class="thumbcaption">
 +
Figure 1.Fluorescence images following the reassembly of sGFP.
 +
</div></div></div></div>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 11: Line 27:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
===Sequence and Features===
 
<partinfo>BBa_K5301014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5301014 SequenceAndFeatures</partinfo>
  

Revision as of 06:28, 1 October 2024


sGFP11 is an engineered version of the final strand of the GFP beta-barrel.


sGFP11 is the smaller fragment of the Split-GFP system, encompassing the final β-strand domain of green fluorescent protein (GFP).The isolated GFP11 fragment does not fluoresce on its own and is typically fused to the C-terminus of the target protein. Upon binding with the GFP1-10 fragment, it facilitates the restoration of GFP's fluorescent activity.


Usage and Biology

In our team's experiments, sGFP1-10 and sGFP11 are individually fused and expressed with the target proteins.We use the self-assembly of GFP1-10 and GFP11 to construct membrane protein dimers.


Characterization

We performed an in vitro mixing and incubation of sGFP1-10 with sGFP11 to achieve their binding. The images captured under a fluorescence microscope are shown in the figure below.


sgfp-fluorescent.png

Figure 1.Fluorescence images following the reassembly of sGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]