Difference between revisions of "Part:BBa K5047039:Design"

 
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===Design Notes===
 
===Design Notes===
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We divided the protein coding sequence of AGO in 3 parts, that we ordered as g-blocks from IDT. The 3 g-blocks facilitate the handling, and assembly of long DNA sequences and were designed to have overlapping regions with each other and with the promoter and terminator.
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===Source===
 
===Source===

Latest revision as of 06:00, 1 October 2024


This sequence enables constitutive Argonaute expression in yeast.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5283
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5191
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3711
    Illegal AgeI site found at 5338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5860


Design Notes

We divided the protein coding sequence of AGO in 3 parts, that we ordered as g-blocks from IDT. The 3 g-blocks facilitate the handling, and assembly of long DNA sequences and were designed to have overlapping regions with each other and with the promoter and terminator.

Source

The promoter comes from the pVW222 plasmid given to us by Prof. Serge Pelet (DMF, university of Lausanne). The protein coding sequence and the terminator were synthetically designed to ensure compatibility and optimal function in yeast.

References