Difference between revisions of "Part:BBa K5186005"

 
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<partinfo>BBa_K5186005 short</partinfo>
 
<partinfo>BBa_K5186005 short</partinfo>
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<i><h2>Description</h2></i>
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“PTac-riboJ-DXS-IspG-IspDF-B0015-PTac-sRNA2(IspH)-B0015” is an expression cassette in <i>E. coli</i> expressing sRNA2(IspH) and overexpress DXS, IspG, IspDF, which leads to <b>the highest yield of HMBPP without affecting bacterial growth</b> in <i>E. coli</i>. With this expression cassette, sRNA2(IspH), DXS, IspG and IspDF can be IPTG-inducibly expressed under the control of pTac and  in all strains of <i>E. coli</i>. It can be used to enhance HMBPP production for mosquito attraction.
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PTac-sRNA2(IspH)-B0015 is an expression cassette in E. coli expressing sRNA2(IspH) (BBa_K5186002), which exhibits the most excellent downregulation effect on IspH gene without affecting bacterial growth in E. coli. With this expression cassette, sRNA2(IspH) can be IPTG-inducibly expressed under the control of pTac-RiboJ (BBa_K3552015) in all strains of E. coli. It can be used to enhance HMBPP production for mosquito attraction.
 
  
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===Usage and Biology===
 
  
 
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Revision as of 05:17, 1 October 2024


PTac-riboJ-DXS-IspG-IspDF-B0015-PTac-sRNA2(IspH)-B0015

Description

“PTac-riboJ-DXS-IspG-IspDF-B0015-PTac-sRNA2(IspH)-B0015” is an expression cassette in E. coli expressing sRNA2(IspH) and overexpress DXS, IspG, IspDF, which leads to the highest yield of HMBPP without affecting bacterial growth in E. coli. With this expression cassette, sRNA2(IspH), DXS, IspG and IspDF can be IPTG-inducibly expressed under the control of pTac and in all strains of E. coli. It can be used to enhance HMBPP production for mosquito attraction.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4325
    Illegal BglII site found at 5541
    Illegal BamHI site found at 3653
    Illegal BamHI site found at 4869
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2102