Difference between revisions of "Part:BBa K173010:Experience"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 5: | Line 4: | ||
===Applications of BBa_K173010=== | ===Applications of BBa_K173010=== | ||
| + | |||
| + | This part was not working in a preliminary induction test. | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | ===='''Materials'''==== | ||
| + | *Long term glycerol stocks were stored at -80°C with a final glycerol concentration of 20% | ||
| + | *Antibiotics were: Ampicillin (Amp) 100 ug/ml, Kanamycin (Kan) 50 ug/ml and Chloramphenicol (Cm) 12.5 ug/ml. All of them were stored at -20°C in 1000x stocks. Amp and Kan were dissolved in water, while Cm was dissolved in ethanol 100%. | ||
| + | *LB medium was prepare with: 1% NaCl, 1% bactotryptone, 0.5% yeast extract. The medium was not buffered with NaOH. | ||
| + | *M9 supplemented medium was prepared according to: [http://openwetware.org/wiki/Knight:M9_supplemented_media Openwetware protocol]. | ||
| + | *3OC6-HSL (Sigma) was dissolved in water and stored at -20°C in a 2mM stock. | ||
| + | *aTc (Clontech) was dissolved in ethanol 50% and stored at -20°C in a 100 ug/ml stock. All the following dilutions were performed in water. | ||
| + | *Ready made IPTG (Sigma) was stored at -20°C in a 200mM stock. | ||
| + | |||
| + | |||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 01:36, 22 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K173010
This part was not working in a preliminary induction test.
Materials
- Long term glycerol stocks were stored at -80°C with a final glycerol concentration of 20%
- Antibiotics were: Ampicillin (Amp) 100 ug/ml, Kanamycin (Kan) 50 ug/ml and Chloramphenicol (Cm) 12.5 ug/ml. All of them were stored at -20°C in 1000x stocks. Amp and Kan were dissolved in water, while Cm was dissolved in ethanol 100%.
- LB medium was prepare with: 1% NaCl, 1% bactotryptone, 0.5% yeast extract. The medium was not buffered with NaOH.
- M9 supplemented medium was prepared according to: [http://openwetware.org/wiki/Knight:M9_supplemented_media Openwetware protocol].
- 3OC6-HSL (Sigma) was dissolved in water and stored at -20°C in a 2mM stock.
- aTc (Clontech) was dissolved in ethanol 50% and stored at -20°C in a 100 ug/ml stock. All the following dilutions were performed in water.
- Ready made IPTG (Sigma) was stored at -20°C in a 200mM stock.
User Reviews
UNIQ4c8d7c2e6687518e-partinfo-00000000-QINU UNIQ4c8d7c2e6687518e-partinfo-00000001-QINU