Difference between revisions of "Part:BBa K5325000:Design"
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===Source=== | ===Source=== | ||
| − | The sequence was taken from Alcanivorax borkumensis genome | + | The sequence was taken from <i>Alcanivorax borkumensis</i> genome |
===References=== | ===References=== | ||
Revision as of 04:09, 1 October 2024
ABO_plaA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 757
Illegal AgeI site found at 673 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Addition of a FLAG tag that aids protein purification and visualization on Western Blot.
DNA sequence was codon optimized for expression in E. coli, which included the change in the start codon from GTG to ATG.
Source
The sequence was taken from Alcanivorax borkumensis genome