Difference between revisions of "Part:BBa K5236022"
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<partinfo>BBa_K5236022 short</partinfo> | <partinfo>BBa_K5236022 short</partinfo> | ||
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| + | This composite part codes for the mutated BhrPETase M57L and expresses the enzyme in the extracellular space of chassis. | ||
T7 promoter: used to regulate gene expression of recombinant proteins. | T7 promoter: used to regulate gene expression of recombinant proteins. | ||
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Lac operator: used to control gene expression by lactose. | Lac operator: used to control gene expression by lactose. | ||
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RBS: the location to bind ribosome. | RBS: the location to bind ribosome. | ||
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BhrPETase M57L: This sequence encodes for a mutated BhrPETase M57L and is derived from Escherichia coli. | BhrPETase M57L: This sequence encodes for a mutated BhrPETase M57L and is derived from Escherichia coli. | ||
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pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane. | pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane. | ||
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6xHis: a tag for the purification of protein which focus in the production of recombinant proteins. | 6xHis: a tag for the purification of protein which focus in the production of recombinant proteins. | ||
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T7 terminator: terminates transcription. | T7 terminator: terminates transcription. | ||
Revision as of 04:03, 1 October 2024
pET28a-pelB-BhrPETase(M57L)-6xHis
This composite part codes for the mutated BhrPETase M57L and expresses the enzyme in the extracellular space of chassis.
T7 promoter: used to regulate gene expression of recombinant proteins.
Lac operator: used to control gene expression by lactose.
RBS: the location to bind ribosome.
BhrPETase M57L: This sequence encodes for a mutated BhrPETase M57L and is derived from Escherichia coli.
pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane.
6xHis: a tag for the purification of protein which focus in the production of recombinant proteins.
T7 terminator: terminates transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 308
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 218
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 912