Difference between revisions of "Part:BBa K5291004"
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NqrF is a subunit of NADH dehydrogenase in <i>P. aeruginosa</i>.
 
NqrF is a subunit of NADH dehydrogenase in <i>P. aeruginosa</i>.
  
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===Usage and Biology===
 
===Usage and Biology===
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The introduction of the pAB1-pS-<i>nqrf</i> plasmid into <i>P. aeruginosa</i> induces the production of NADH dehydrogenase, which catalyzes the transformation of NADH into and electrons, increasing the amount of electrons in <i>P. aeruginosa</i> and thus promoting electron shuttles (PYO), which indirectly transmit electrons to <i>R. palustris</i>. We have consturcted the plasmid pAB1-pS-<i>nqrf</i>.<br><br>
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<html><img width = "650" src="https://static.igem.wiki/teams/5291/images/part-zwd/1-pab1-ps-nqrf-map.png" /></html>
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<br><b>Fig.1 Plasmid construction of pAB1-pS-<i>nqrf</i>.</b><br>
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<html><img width = "650" src="https://static.igem.wiki/teams/5291/images/part-zwd/5-pab-ps-nqrf-p.png" /></html>
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<br><b>Fig.2 The agarose gel electrophoresis analysis of pAB1-pS-<i>nqrf</i>.</b><br><br>
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Revision as of 03:19, 1 October 2024


NqrF

NqrF is a subunit of NADH dehydrogenase in P. aeruginosa.

Usage and Biology

The introduction of the pAB1-pS-nqrf plasmid into P. aeruginosa induces the production of NADH dehydrogenase, which catalyzes the transformation of NADH into and electrons, increasing the amount of electrons in P. aeruginosa and thus promoting electron shuttles (PYO), which indirectly transmit electrons to R. palustris. We have consturcted the plasmid pAB1-pS-nqrf.


Fig.1 Plasmid construction of pAB1-pS-nqrf.

Fig.2 The agarose gel electrophoresis analysis of pAB1-pS-nqrf.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 976
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 976
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 976
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 976
    Illegal NgoMIV site found at 150
    Illegal NgoMIV site found at 154
    Illegal NgoMIV site found at 493
    Illegal NgoMIV site found at 847
    Illegal NgoMIV site found at 890
    Illegal AgeI site found at 1054
  • 1000
    COMPATIBLE WITH RFC[1000]