Difference between revisions of "Part:BBa K5236014"
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T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins. | T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins. | ||
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Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG. | Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG. | ||
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RBS: ribosome binding site. | RBS: ribosome binding site. | ||
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IsPETase M10L: This sequence encodes for a mutated IsPETase M10L and is derived from Escherichia coli. | IsPETase M10L: This sequence encodes for a mutated IsPETase M10L and is derived from Escherichia coli. | ||
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pelB: The sequence encodes a signal peptide that enables secretory expression of PETase. | pelB: The sequence encodes a signal peptide that enables secretory expression of PETase. | ||
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6xHis: A label for protein purification | 6xHis: A label for protein purification | ||
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T7 terminator: Terminates transcription. | T7 terminator: Terminates transcription. | ||
Revision as of 02:43, 1 October 2024
pET28a-pelB-IsPETase(M10L)-6xHis
T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins.
Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG.
RBS: ribosome binding site.
IsPETase M10L: This sequence encodes for a mutated IsPETase M10L and is derived from Escherichia coli.
pelB: The sequence encodes a signal peptide that enables secretory expression of PETase.
6xHis: A label for protein purification
T7 terminator: Terminates transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1005