Difference between revisions of "Part:BBa K5301022"
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− | This pathway | + | This pathway achieving self-cyclization of NW50 to construct nanodiscs. |
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Revision as of 02:19, 1 October 2024
SpyCatcher-spNW50-SpyTag achieving self-cyclization of MSP nanodisc.
This pathway achieving self-cyclization of NW50 to construct nanodiscs.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 565
Illegal EcoRI site found at 1645
Illegal SpeI site found at 517
Illegal PstI site found at 733
Illegal PstI site found at 1273
Illegal PstI site found at 1306
Illegal PstI site found at 1813 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 565
Illegal EcoRI site found at 1645
Illegal NheI site found at 133
Illegal NheI site found at 2061
Illegal SpeI site found at 517
Illegal PstI site found at 733
Illegal PstI site found at 1273
Illegal PstI site found at 1306
Illegal PstI site found at 1813 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 565
Illegal EcoRI site found at 1645
Illegal BglII site found at 993
Illegal BglII site found at 1533
Illegal BglII site found at 1701
Illegal BamHI site found at 166 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 565
Illegal EcoRI site found at 1645
Illegal SpeI site found at 517
Illegal PstI site found at 733
Illegal PstI site found at 1273
Illegal PstI site found at 1306
Illegal PstI site found at 1813 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 565
Illegal EcoRI site found at 1645
Illegal SpeI site found at 517
Illegal PstI site found at 733
Illegal PstI site found at 1273
Illegal PstI site found at 1306
Illegal PstI site found at 1813 - 1000COMPATIBLE WITH RFC[1000]