Difference between revisions of "Part:BBa K243033:Design"
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<partinfo>BBa_K243033 short</partinfo> | <partinfo>BBa_K243033 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | [https://static.igem.org/mediawiki/parts/b/bc/Freiburg09_pEX%21.txt Commented GenBank file] | |
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===Source=== | ===Source=== |
Revision as of 01:13, 22 October 2009
pEX
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 2734
Illegal XbaI site found at 1523 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 2735
Illegal PstI site found at 2749
Illegal NotI site found at 2742 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1808
- 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 2735
Illegal XbaI site found at 1523 - 25INCOMPATIBLE WITH RFC[25]Illegal suffix found in sequence at 2725
Illegal XbaI site found at 1523
Illegal NgoMIV site found at 1531 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3800
Illegal SapI.rc site found at 4799
Design Notes
Source
By adding additional restriction sites in pMAL we create our expression vector.