To create an biosensor capable of detecting the anti-inflammatory drug ibuprofen, the FRET-based sensor system [https://parts.igem.org/Part:BBa_K1907000 (BBa_K4447004)] that we propose by changing the gene[https://parts.igem.org/Part:BBa_K1907000 (BBa_K4447001)], for the gene[https://parts.igem.org/Part:BBa_K5439005 (BBa_K5439005)]. By changing the genes, our composite part can detect ibuprofen from water bodies. Ibuprofen is an anti-inflammatory treatment drug widely used in the world that can be bought without any necessary prescription. This makes ibuprofen a drug that everyone can consume easily, bringing problems because its disposal makes it an emerging contaminant in water bodies (Jan-Roblero, J., & Cruz-Maya, J. A., 2023). FRET (fluorescence resonance energy transfer) is a biosensor technique that detects biomolecules without modifying them. It relies on the proximity of fluorophore molecules to trigger fluorescence. This non-radiative process allows for sensitive and specific detection of environmental changes and biomolecule interactions. FRET biosensors are safe and versatile, able to detect protein-protein interactions, pH changes, enzyme activity, and more. They provide a reliable means of monitoring various biomolecular activities without the need for genetic modifications, making them valuable tools in research and diagnostics (Kumar-Verma, A., <i>et al.</i>, 2023).