Difference between revisions of "Part:BBa K5439006"
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FRET-based sensor system for the detection of ibuprofen that consists of long-chain fatty acid CoA ligase from Sphingomonas spp.[https://parts.igem.org/Part:BBa_K5439005 (BBa_K5439005)],an enzyme that catalyzes the conversion of ibuprofen into isobutylcatechol, flanked by two fluorescent proteins: ECFP[https://parts.igem.org/Part:BBa_K1159302 (BBa_K1159302)]as energy donor and mVenus[https://parts.igem.org/Part:BBa_K1907000 (BBa_K1907000)]as an energy acceptor  
 
FRET-based sensor system for the detection of ibuprofen that consists of long-chain fatty acid CoA ligase from Sphingomonas spp.[https://parts.igem.org/Part:BBa_K5439005 (BBa_K5439005)],an enzyme that catalyzes the conversion of ibuprofen into isobutylcatechol, flanked by two fluorescent proteins: ECFP[https://parts.igem.org/Part:BBa_K1159302 (BBa_K1159302)]as energy donor and mVenus[https://parts.igem.org/Part:BBa_K1907000 (BBa_K1907000)]as an energy acceptor  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5439006 SequenceAndFeatures</partinfo>
 
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Revision as of 00:40, 1 October 2024


FRET-based system for the detection of ibuprofen

FRET-based sensor system for the detection of ibuprofen that consists of long-chain fatty acid CoA ligase from Sphingomonas spp.(BBa_K5439005),an enzyme that catalyzes the conversion of ibuprofen into isobutylcatechol, flanked by two fluorescent proteins: ECFP(BBa_K1159302)as energy donor and mVenus(BBa_K1907000)as an energy acceptor

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2174
    Illegal BsaI.rc site found at 2959

Usage and Biology

To create an biosensor capable of detecting the anti-inflammatory drug ibuprofen, the FRET-based sensor system (BBa_K4447004) changing the middle part of the construct instead of the gene (BBa_K4447001), being the gene of interest (BBa_K5439005); changing the