Difference between revisions of "Part:BBa K200022:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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===User Reviews===
 
===User Reviews===
 
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<partinfo>BBa_K200022 AddReview number</partinfo>
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<partinfo>BBa_K200022 Review 1</partinfo>
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<I>Username</I>
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<I>Kun</I>
 
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Enter the review inofrmation here.
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Cells were grown at the 2 different temperatures of 28°C and 42°C. There was in addition a set of cells that were shifted from 28°C to 42°C so that we could characterise the change in GFP fluorescence during the transition between temperatures.<br>
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<br>
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In order to characterize the thermoinducible promoter properly, we have used 3 sets of control cells (not shown in graph): <br>
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*Positive control cells: Containing the BBa_I13522, acting as a baseline comparison by constitutively expressing GFP.
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*Negative control cells: These contain the thermoinducible promoter on its own ( BBa_K098995) with no GFP attached to it. These serve as cells without any fluorescence.
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*Cells shifted from 28 to 42 degrees. This characterises the transition stage.
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<br>
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[[Image:II09_Thermoconstruct_main2.jpg|500px]]<br>
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The characterization of the thermoinducible promoter is as follows:<br>
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*At 28 ºC, there is no fluorescence (GFP) output because its expression is repressed
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*At 42 ºC, we can observe an increase in fluorescence (GFP) output,as its expression is no longer repressed
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Hence, we can conclude that an increase in temperature de-represses the expression of the thermoinducible system.<br>
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As part of Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London| <i>The E.ncapsulator</i>] project, we characterised this part using the composite [https://parts.igem.org/Part:BBa_K200022| BBa_K200022].<br>
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Latest revision as of 01:04, 22 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K200022

User Reviews

UNIQ8a476455494e4937-partinfo-00000000-QINU

BBa_K200022 Review 1 Not understood Kun

Cells were grown at the 2 different temperatures of 28°C and 42°C. There was in addition a set of cells that were shifted from 28°C to 42°C so that we could characterise the change in GFP fluorescence during the transition between temperatures.

In order to characterize the thermoinducible promoter properly, we have used 3 sets of control cells (not shown in graph):

  • Positive control cells: Containing the BBa_I13522, acting as a baseline comparison by constitutively expressing GFP.
  • Negative control cells: These contain the thermoinducible promoter on its own ( BBa_K098995) with no GFP attached to it. These serve as cells without any fluorescence.
  • Cells shifted from 28 to 42 degrees. This characterises the transition stage.


II09 Thermoconstruct main2.jpg
The characterization of the thermoinducible promoter is as follows:

  • At 28 ºC, there is no fluorescence (GFP) output because its expression is repressed
  • At 42 ºC, we can observe an increase in fluorescence (GFP) output,as its expression is no longer repressed

Hence, we can conclude that an increase in temperature de-represses the expression of the thermoinducible system.

As part of Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London| The E.ncapsulator] project, we characterised this part using the composite BBa_K200022.

;

UNIQ8a476455494e4937-partinfo-00000002-QINU