Difference between revisions of "Part:BBa K098995:Experience"

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<I>Kun Xue</I>
 
<I>Kun Xue</I>
 
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In order to characterize the thermoinducible promoter properly, we have used 3 sets of control cells (not shown in graph): <br>
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*Positive control cells: Containing the BBa_I13522, acting as a baseline comparison by constitutively expressing GFP.
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*Negative control cells: These contain the thermoinducible promoter on its own ( BBa_K098995) with no GFP attached to it. These serve as cells without any fluorescence.
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*Cells shifted from 28 to 42 degrees. This characterises the transition stage.
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<br>
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[[Image:II09_Thermoconstruct_main2.jpg|500px]]<br>
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The characterization of the thermoinducible promoter is as follows:<br>
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*At 28 ºC, there is no fluorescence (GFP) output because its expression is repressed
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*At 42 ºC, we can observe an increase in fluorescence (GFP) output,as its expression is no longer repressed
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Hence, we can conclude that an increase in temperature de-represses the expression of the thermoinducible system.<br>
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As part of Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London| <i>The E.ncapsulator</i>] project, we characterised this part using the composite [https://parts.igem.org/Part:BBa_K200022| BBa_K200022].<br>
 
As part of Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London| <i>The E.ncapsulator</i>] project, we characterised this part using the composite [https://parts.igem.org/Part:BBa_K200022| BBa_K200022].<br>
  

Revision as of 01:04, 22 October 2009

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Applications of BBa_K098995

User Reviews

UNIQb9da1e56c0b3c1b5-partinfo-00000000-QINU

BBa_K098995 Kun Xue

In order to characterize the thermoinducible promoter properly, we have used 3 sets of control cells (not shown in graph):

  • Positive control cells: Containing the BBa_I13522, acting as a baseline comparison by constitutively expressing GFP.
  • Negative control cells: These contain the thermoinducible promoter on its own ( BBa_K098995) with no GFP attached to it. These serve as cells without any fluorescence.
  • Cells shifted from 28 to 42 degrees. This characterises the transition stage.


II09 Thermoconstruct main2.jpg
The characterization of the thermoinducible promoter is as follows:

  • At 28 ºC, there is no fluorescence (GFP) output because its expression is repressed
  • At 42 ºC, we can observe an increase in fluorescence (GFP) output,as its expression is no longer repressed

Hence, we can conclude that an increase in temperature de-represses the expression of the thermoinducible system.

As part of Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London| The E.ncapsulator] project, we characterised this part using the composite BBa_K200022.



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UNIQb9da1e56c0b3c1b5-partinfo-00000002-QINU