Difference between revisions of "Part:BBa K5102072:Design"

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(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The composite part consists of: CMVenhancer, CMVpromotor, T7 promoter, recording tape 1.0, T2A, miRFP670nano3, P2A, eUnaG, T2A,  mScarlet3, P2A, eUnaG, T2A, mTagBFP2, P2A, eUnaG, human beta-globin 3'UTR, PP7, T7 terminator, WPRE, SV40 polyA.
  
 
===Source===
 
===Source===

Revision as of 23:33, 30 September 2024


pRAM_ProgRAM-recording-tape1.0


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 18
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2494
    Illegal BamHI site found at 3554
    Illegal XhoI site found at 2563
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 18
    Illegal NgoMIV site found at 4899
    Illegal AgeI site found at 3645
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The composite part consists of: CMVenhancer, CMVpromotor, T7 promoter, recording tape 1.0, T2A, miRFP670nano3, P2A, eUnaG, T2A, mScarlet3, P2A, eUnaG, T2A, mTagBFP2, P2A, eUnaG, human beta-globin 3'UTR, PP7, T7 terminator, WPRE, SV40 polyA.

Source

The composite was assembled from gblocks and oligos provided by a DNA synthesis provider.

References