Difference between revisions of "Part:BBa K243009:Design"
(→Design Notes) |
|||
| Line 8: | Line 8: | ||
The length of this linker is useful to produce a connection to another part and produce great distance between two parts. The long distance produced by the linker reduces possible sterical interferences and increases the distance from the protein Fok_i to the DNA when it is linked to a DNA binding protein. We had to prove the choice of distance between two parts and the distance between our protein domain Fok_a and Fok_i. <br> | The length of this linker is useful to produce a connection to another part and produce great distance between two parts. The long distance produced by the linker reduces possible sterical interferences and increases the distance from the protein Fok_i to the DNA when it is linked to a DNA binding protein. We had to prove the choice of distance between two parts and the distance between our protein domain Fok_a and Fok_i. <br> | ||
Designed according to the [https://parts.igem.org/Assembly_standard_25 RFC 25]. | Designed according to the [https://parts.igem.org/Assembly_standard_25 RFC 25]. | ||
| + | |||
| + | [https://static.igem.org/mediawiki/parts/b/b8/Freiburg09_LL_Foki.txt Commented GenBank file] | ||
===Source=== | ===Source=== | ||
Latest revision as of 00:50, 22 October 2009
Long Linker-Fok_i
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The length of this linker is useful to produce a connection to another part and produce great distance between two parts. The long distance produced by the linker reduces possible sterical interferences and increases the distance from the protein Fok_i to the DNA when it is linked to a DNA binding protein. We had to prove the choice of distance between two parts and the distance between our protein domain Fok_a and Fok_i.
Designed according to the RFC 25.
Source
Combinated by cloning.