Difference between revisions of "Part:BBa K187424"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K187424 short</partinfo> | <partinfo>BBa_K187424 short</partinfo> | ||
| − | The | + | The origin can be amplified from pMB1 using this primer in conjunction with the Origin AB reverse primer. A high copy origin can be produced with A and B ends compatible with the BioBytes assembly method. Origins cannot be cloned into pAB and pBA using the standard BioBytes method because the presence of two origins will destabilize the plasmid. Some thymine bases in the above sequence must be replaced with uracil for correct function. Actual sequence is as follows: |
5' AGGAAGUACGGUGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGC 3' | 5' AGGAAGUACGGUGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGC 3' | ||
Latest revision as of 00:49, 22 October 2009
Origin in AB, Forward primer
The origin can be amplified from pMB1 using this primer in conjunction with the Origin AB reverse primer. A high copy origin can be produced with A and B ends compatible with the BioBytes assembly method. Origins cannot be cloned into pAB and pBA using the standard BioBytes method because the presence of two origins will destabilize the plasmid. Some thymine bases in the above sequence must be replaced with uracil for correct function. Actual sequence is as follows:
5' AGGAAGUACGGUGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGC 3'
This part will allow amplification of a high copy origin to allow proliferation of an artificial BioBytes plasmid. See BBF RFC#47 for information on the BioBytes method.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]