Difference between revisions of "Part:BBa K5102069"

 
 
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The CMV enhancer is a regulatory element derived from the cytomegalovirus (CMV) immediate-early promoter. It is widely used in synthetic biology and gene expression systems to significantly boost the transcriptional activity of promoters to which it is linked.  
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The WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element) is a sequence derived from the woodchuck hepatitis virus, commonly used in gene expression systems to enhance the stability and translation of mRNA. When incorporated into plasmids or viral vectors, WPRE improves the nuclear export and cytoplasmic accumulation of transgene mRNA, leading to increased protein expression levels.  
  
 
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Latest revision as of 21:17, 30 September 2024


Woodchuck posttranscriptional regulatory element (WPRE)

The WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element) is a sequence derived from the woodchuck hepatitis virus, commonly used in gene expression systems to enhance the stability and translation of mRNA. When incorporated into plasmids or viral vectors, WPRE improves the nuclear export and cytoplasmic accumulation of transgene mRNA, leading to increased protein expression levels.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 518
  • 1000
    COMPATIBLE WITH RFC[1000]