Difference between revisions of "Part:BBa K5508008:Design"

 
 
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===Design Notes===
 
===Design Notes===
 
We ligated the fluorescent protein gene mCherry to HsLF so that we could determine whether HsLF was successfully expressed by the fluorescent protein expression system.
 
We ligated the fluorescent protein gene mCherry to HsLF so that we could determine whether HsLF was successfully expressed by the fluorescent protein expression system.
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    .bild {max-width: 35% ; height: auto;}
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<p>
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  <img class="bild" src="https://static.igem.wiki/teams/5508/hangzhoumedx-parts/28.png">
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  <div class="unterschrift"><b> pESC-HsLF-mCherry</b>
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  </div>
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</p>
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</html>
  
  

Latest revision as of 21:02, 30 September 2024


Gal1,10-HsLF-mCherry-CYC1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1833
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1833
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2248
    Illegal BglII site found at 2372
    Illegal BglII site found at 3359
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1833
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1833
    Illegal NgoMIV site found at 3215
    Illegal AgeI site found at 1247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2712


Design Notes

We ligated the fluorescent protein gene mCherry to HsLF so that we could determine whether HsLF was successfully expressed by the fluorescent protein expression system.

pESC-HsLF-mCherry


Source

None

References