Difference between revisions of "Part:BBa K5508001:Design"

 
 
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===Design Notes===
 
===Design Notes===
 
The BBa_K5508001(HsLF gene) was placed under the control of BBa_K5477005(bidirectional promoters Gal1,10), and the BBa_K3384311(CYC1 terminator) was responsible for terminating its transcription for efficient expression of the lactoferrin gene.
 
The BBa_K5508001(HsLF gene) was placed under the control of BBa_K5477005(bidirectional promoters Gal1,10), and the BBa_K3384311(CYC1 terminator) was responsible for terminating its transcription for efficient expression of the lactoferrin gene.
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<p>
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  <img class="bild" src="https://static.igem.wiki/teams/5508/hangzhoumedx-parts/1.png">
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  <div class="unterschrift"><b> pESC-HsLf</b>
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</p>
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  <img class="bild" src="https://static.igem.wiki/teams/5508/hangzhoumedx-parts/2.png">
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  <div class="unterschrift"><b> GAL1,10-HsLF-CYC1</b>
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  </div>
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</p>
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</html>
 
===Source===
 
===Source===
  

Latest revision as of 20:22, 30 September 2024


GAL1,10-HsLF-CYC1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 960
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 960
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1375
    Illegal BglII site found at 1499
    Illegal BglII site found at 2486
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 960
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 960
    Illegal NgoMIV site found at 2342
    Illegal AgeI site found at 374
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1839


Design Notes

The BBa_K5508001(HsLF gene) was placed under the control of BBa_K5477005(bidirectional promoters Gal1,10), and the BBa_K3384311(CYC1 terminator) was responsible for terminating its transcription for efficient expression of the lactoferrin gene.

pESC-HsLf

GAL1,10-HsLF-CYC1

Source

None

References