Difference between revisions of "Part:BBa K257011:Experience"
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===Applications of BBa_K257011=== | ===Applications of BBa_K257011=== | ||
| − | + | Pbad-clyA-RFP on arabinose medium | |
| − | + | [[Image:TolA clyA Ara 01.jpg]] | |
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| + | Pbad-clyA-RFP on LB medium (without arabinose) | ||
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| + | [[Image:TolA clyA LB 01.jpg]] | ||
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| + | Pfec-RFP on medium with citrate (without clyA, fluorescence isn't localize on the membrane exclusively) | ||
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| + | [[Image:Citrate30.jpg]] | ||
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| + | On the first image, we observed fluorescence localized to the membrane when growing bacteria transformed with PBad-ClyA-RFP on a medium with arabinose. We can compare it to 2 others images from microcoscopy : with Pbad-clyA-RFP without arabinose, the fluorescent is less intense (pBad isn't repressed so there is little expression of clyA-RFP). The third image demonstrate that without clyA, RFP isn't localize exclusively in the membrane (here, it's an example with Bba_K257023) | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 00:31, 22 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K257011
Pbad-clyA-RFP on arabinose medium
Pbad-clyA-RFP on LB medium (without arabinose)
Pfec-RFP on medium with citrate (without clyA, fluorescence isn't localize on the membrane exclusively)
On the first image, we observed fluorescence localized to the membrane when growing bacteria transformed with PBad-ClyA-RFP on a medium with arabinose. We can compare it to 2 others images from microcoscopy : with Pbad-clyA-RFP without arabinose, the fluorescent is less intense (pBad isn't repressed so there is little expression of clyA-RFP). The third image demonstrate that without clyA, RFP isn't localize exclusively in the membrane (here, it's an example with Bba_K257023)
User Reviews
UNIQ904334fe36af223f-partinfo-00000000-QINU UNIQ904334fe36af223f-partinfo-00000001-QINU