Difference between revisions of "Part:BBa K5322013"

 
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<partinfo>BBa_K5322013 short</partinfo>
  
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==Usage and Biology==
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<p>
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Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7
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The pET29a vector is used for high-level expression in E. coli. The expression of SOD-1 is controlled by the NO-responsive promoter SoxR/SoxS. The ribosome binding site (RBS) ensures efficient translation of mRNA, while the T7 terminator provides a clean and efficient end for transcription. This system is designed to express SOD only in the intestinal inflammation site and exert its antioxidant effect.
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</P>
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==Structural design==
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<p>
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Our team designed the gene circuit shown in the figure below: pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7.
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  .center-img {
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    text-align:center;
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<div class="center-img">
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<img src="https://static.igem.wiki/teams/5322/wet-lab/soxs-opju.png" alt="pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7" width="300">
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<p align="center"><b>Figure 1-1</b> gene circuit pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7</p>
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</div>
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==Construction of the plasmid==
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<html>
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<p>
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Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7
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The pET29a vector is used for high-level expression in E. coli. The expression of SOD-1 is controlled by the NO-responsive promoter SoxR/SoxS. The ribosome binding site (RBS) ensures efficient translation of mRNA, while the T7 terminator provides a clean and efficient end for transcription. This system is designed to express SOD only in the intestinal inflammation site and exert its antioxidant effect.
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</p>
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<style>
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  .center-img {
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    text-align:center;
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  }
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<div class="center-img">
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<img src="https://static.igem.wiki/teams/5322/wet-lab/20-pet29a-j23119-rbs-mfp3-t7.png" alt="pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7" width="300">
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<p align="center"><b>Figure 2-1</b> Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7</p>
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</div>
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==Sequence and Features==
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<partinfo>BBa_K5101013 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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==Functional Parameters==
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<partinfo>BBa_K5101013 parameters</partinfo>
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Revision as of 18:43, 30 September 2024

pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7.

Usage and Biology

Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7 The pET29a vector is used for high-level expression in E. coli. The expression of SOD-1 is controlled by the NO-responsive promoter SoxR/SoxS. The ribosome binding site (RBS) ensures efficient translation of mRNA, while the T7 terminator provides a clean and efficient end for transcription. This system is designed to express SOD only in the intestinal inflammation site and exert its antioxidant effect.

Structural design

Our team designed the gene circuit shown in the figure below: pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7.

pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7

Figure 1-1 gene circuit pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7

==Construction of the plasmid==

Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7 The pET29a vector is used for high-level expression in E. coli. The expression of SOD-1 is controlled by the NO-responsive promoter SoxR/SoxS. The ribosome binding site (RBS) ensures efficient translation of mRNA, while the T7 terminator provides a clean and efficient end for transcription. This system is designed to express SOD only in the intestinal inflammation site and exert its antioxidant effect.

pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7

Figure 2-1 Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7

==Sequence and Features== BBa_K5101013 SequenceAndFeatures