Difference between revisions of "Part:BBa K200018:Experience"
(→‎User Reviews)
Line 27: Line 27:
 
<I>Kun Xue</I>
 
<I>Kun Xue</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
−
[[Image:
+
[[Image:II09_CRP-GFP fluor different media.jpg|500px|right]]
 +
Cells with BBa_K200018 were grown overnight in various different media, and the GFP fluorescence was measured. <br>
 +
<br>
 +
After overnight culture, the corrected fluorescence of glucose is almost negligible, showing that glucose represses the PcstA promoter strongly.  <br>
 +
<br>
 +
For all the other secondary carbon sources, 10% Casamino Acids in M9 shows the highest corrected fluorescence at 22000.<br>
 +
[[Image:II09_CRP-GFP abs different media.jpg|500px|right]]
 +
For effective gene expression, not only must the promoter activity be strong, but there must also be enough cells expressing the genes.  Therefore, OD600 is also a critical factor.<br>
 +
<br>
 +
Due to the <b>exceedingly low OD600</b> in 0.5% Lactose in M9, the medium cannot be used even though it induces relatively high fluorescence.<br>
 +
<br>
 +
For more information, go to the Imperial iGEM 2009 <i>E.ncapsulator</i> project page on
 +
[http://2009.igem.org/Team:Imperial_College_London/Wetlab/Results/CRP_and_Media BBa_K200018 testing results] <br>
 +
 
 +
 
 +
 
 
|};
 
|};
  
 
<partinfo>BBa_K200018 EndReviews</partinfo>
 
<partinfo>BBa_K200018 EndReviews</partinfo>

Revision as of 00:27, 22 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K200018

User Reviews

UNIQ631d9c7ec900f259-partinfo-00000000-QINU UNIQ631d9c7ec900f259-partinfo-00000001-QINU

UNIQ631d9c7ec900f259-partinfo-00000002-QINU

BBa_K200018 1 Not understood Kun Xue

II09 CRP-GFP fluor different media.jpg

Cells with BBa_K200018 were grown overnight in various different media, and the GFP fluorescence was measured.

After overnight culture, the corrected fluorescence of glucose is almost negligible, showing that glucose represses the PcstA promoter strongly.

For all the other secondary carbon sources, 10% Casamino Acids in M9 shows the highest corrected fluorescence at 22000.

II09 CRP-GFP abs different media.jpg

For effective gene expression, not only must the promoter activity be strong, but there must also be enough cells expressing the genes. Therefore, OD600 is also a critical factor.

Due to the exceedingly low OD600 in 0.5% Lactose in M9, the medium cannot be used even though it induces relatively high fluorescence.

For more information, go to the Imperial iGEM 2009 E.ncapsulator project page on [http://2009.igem.org/Team:Imperial_College_London/Wetlab/Results/CRP_and_Media BBa_K200018 testing results]


;

UNIQ631d9c7ec900f259-partinfo-00000004-QINU