Difference between revisions of "Part:BBa K257011"
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We observed fluorescence localized to the membrane when growing bacteria transformed with PBad-ClyA-RFP on a medium with arabinose. | We observed fluorescence localized to the membrane when growing bacteria transformed with PBad-ClyA-RFP on a medium with arabinose. |
Revision as of 00:18, 22 October 2009
PBad + clyA_CTerm + RFP_NTerm
This part allows us to express the fusion protein clyA-RFP. In a mutant strain which constitutively produce vesicles, the fusion protein inserted in the outer membrane of the bacteria will be incorporated, during vesiculation, into the lumen of the vesicles. The promoter pBad is inductible with L-arabinose.
Experience
Pbad-clyA-RFP on arabinose medium
Pbad-clyA-RFP on LB medium (without arabinose)
Pfec-RFP on medium with citrate (without clyA, fluorescence isn't localize on the membrane exclusively)
We observed fluorescence localized to the membrane when growing bacteria transformed with PBad-ClyA-RFP on a medium with arabinose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1302
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 2712
Illegal AgeI site found at 2824 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961