Difference between revisions of "Part:BBa K243011"
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K243011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K243011 SequenceAndFeatures</partinfo>
  

Latest revision as of 00:15, 22 October 2009

Strep-DigA-Long Linker-Fok_i

This part unites some of our basic parts. The combination of a StrepTag, an Anticalin-Tag and the linked protein domain Fok_i makes it possible to purify and detect the function of the protein. The DigoxigeninA tag allows the coupling to a digoxigenin linked oligonucleotide.


Usage and Biology

This composite part is one part of our universal endonuclease and needs another composite part like BBa_K243024 to build a functional heterodimer. The DigA tag leads the part to DNA which is hybridized with a Digoxigenin labeled oligonucleotide. The long linker creates a distance of 36bp between the DigA and the linked Fok_i protein domain. The StrepTag serves as a purification tag for Streptavidin column purification.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]