Difference between revisions of "Part:BBa K243025:Design"

(→‎Design Notes)
(→‎Design Notes)
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The linker length and the choice of the purification tag in combination with the anticalin tag allowed us several combination possibilities, which we had to prove to achieve the best combination.
 
The linker length and the choice of the purification tag in combination with the anticalin tag allowed us several combination possibilities, which we had to prove to achieve the best combination.
  
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[https://static.igem.org/mediawiki/parts/d/dd/Freiburg09_Strep-FluA-ML-Fok_is.txt  
+
[https://static.igem.org/mediawiki/parts/d/dd/Freiburg09_Strep-FluA-ML-Fok_is.txt Commented GenBank file]
−
Commented GenBank file]
+
  
 
===Source===
 
===Source===

Revision as of 00:06, 22 October 2009

Strep-FluA-Middle Linker-Fok_i


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The linker length and the choice of the purification tag in combination with the anticalin tag allowed us several combination possibilities, which we had to prove to achieve the best combination.

Commented GenBank file

Source

Combined by serial clonig steps.

References