Difference between revisions of "Part:BBa K5322034"

Revision as of 15:00, 30 September 2024

Programmed Cell Death 1 Ligand 1 Functional Domain [Mus musculus]

Usage and Biology

The full-length mouse PD-L1 protein has a large molecular weight and a complex structure. Therefore, we referred to the descriptions of mouse PD-L1 in the NCBI database (ADK70951.1, ADK70950.1, Q9NZQ7.1, AAH66841.1) and truncated the Programmed Cell Death 1 Ligand 1 [Mus musculus] (BBa_K5322033). The functional domain—the immunoglobulin variable (IgV) domain—corresponds to residues 20 to 130 in the 290 amino acid sequence. To ensure that the functionality of the truncated protein is preserved, we retained the first 19 amino acids and the last 20 amino acids (131-150), aiming to minimize any impact on the IgV structure.

Protein Structure Prediction

2.1 AlphaFold 2 Prediction Results

For the PD-L1 protein, we utilized AlphaFold 2 from Google DeepMind for protein structure prediction, aiming to gain a deeper understanding of PD-L1.

AlphaFold 2 Prediction Results:

Figure 2-1 AlphaFold 2 Prediction Results

2.2 AlphaFold 3 Prediction Results

With the release of AlphaFold 3, the accuracy of protein structure prediction has been further improved. Therefore, we conducted another round of protein structure prediction.

AlphaFold 3 Prediction Results:

Figure 2-2 AlphaFold 3 Prediction Results

Sequence and Features