Difference between revisions of "Part:BBa K5097011"
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<partinfo>BBa_K5097011 short</partinfo> | <partinfo>BBa_K5097011 short</partinfo> | ||
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This composite part functions as an acid sensing, base producing part. It contains the coding sequences speA and speB, the two enzymes involved in the conversion of arginine to putrescine (Morris., et al 1969). These genes, when expressed, will produce putrescine, a base. Expression of these genes is controlled by pASR, an acid sensitive promoter sequence from the parts repository. The 2024 Oneonta iGEM team designed this sequence for use in pH adjusting devices for use in the remediation of wastewater from the manufacturing of microprocessors. | This composite part functions as an acid sensing, base producing part. It contains the coding sequences speA and speB, the two enzymes involved in the conversion of arginine to putrescine (Morris., et al 1969). These genes, when expressed, will produce putrescine, a base. Expression of these genes is controlled by pASR, an acid sensitive promoter sequence from the parts repository. The 2024 Oneonta iGEM team designed this sequence for use in pH adjusting devices for use in the remediation of wastewater from the manufacturing of microprocessors. | ||
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Revision as of 14:55, 30 September 2024
Acid-sensor, Base-producer pASR-PUT1
Usage and Biology
This composite part functions as an acid sensing, base producing part. It contains the coding sequences speA and speB, the two enzymes involved in the conversion of arginine to putrescine (Morris., et al 1969). These genes, when expressed, will produce putrescine, a base. Expression of these genes is controlled by pASR, an acid sensitive promoter sequence from the parts repository. The 2024 Oneonta iGEM team designed this sequence for use in pH adjusting devices for use in the remediation of wastewater from the manufacturing of microprocessors.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2930
Illegal BamHI site found at 1541
Illegal BamHI site found at 2843 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1319
Illegal AgeI site found at 2865 - 1000COMPATIBLE WITH RFC[1000]