Difference between revisions of "Part:BBa K5097010"

Revision as of 14:43, 30 September 2024

SpeB (Agmatinase)

Usage and Biology

This part codes for the SpeB gene from E.coli. The gene codes for agmatine ureohydrolase. The enzyme is responsible for the catabolism of agmatine. It converts agmatine into urea and putrescine, and is the second step in the metabolic pathway that converts arginine into putrescine (Morris., et 1969). The 2024 Oneonta iGEM team designed the sequence for use in a base producing circuit. When co-expressed with SpeA (BBa_K5097010) it will construct the pathway from turning arginine into putrescine. Acidic conditions will be neutralized with the production of putrescine.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 789
Illegal BamHI site found at 702
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 724
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K5097010 short</partinfo>
+===Usage and Biology===
 This part codes for the SpeB gene from E.coli. The gene codes for agmatine ureohydrolase. The enzyme is responsible for the catabolism of agmatine. It converts agmatine into urea and putrescine, and is the second step in the metabolic pathway that converts arginine into putrescine (Morris., et 1969). The 2024 Oneonta iGEM team designed the sequence for use in a base producing circuit. When co-expressed with SpeA (BBa_K5097010) it will construct the pathway from turning arginine into putrescine. Acidic conditions will be neutralized with the production of putrescine.
@@ Line 7: / Line 9: @@
 <!-- Add more about the biology of this part here
-===Usage and Biology===
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