Difference between revisions of "Part:BBa K5348002"

 
 
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<partinfo>BBa_K5348002 short</partinfo>
 
<partinfo>BBa_K5348002 short</partinfo>
  
 
RBS mutants with a 108-fold reduction in intensity over B0034.
 
RBS mutants with a 108-fold reduction in intensity over B0034.
  
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===Usage and Biology===
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===Functional Parameters===
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<partinfo>BBa_K5348002 parameters</partinfo>
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    <title>B0034-mutant 2 (RBS2) - BBa_K5348002</title>
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    <h2>B0034-mutant 2 (RBS2) - BBa_K5348002</h2>
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    <h3>Profile</h3>
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    <table>
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        <tr>
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            <th>Name</th>
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            <td>B0034-mutant 2 (RBS2)</td>
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        </tr>
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        <tr>
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            <th>Base Pairs</th>
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            <td>12 bp</td>
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        </tr>
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        <tr>
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            <th>Sequence</th>
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            <td>GCTCCATCCCCG</td>
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        </tr>
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        <tr>
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            <th>Origin</th>
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            <td>Synthesized</td>
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    <h3>Properties</h3>
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    <p>
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        The ribosome binding site (RBS) is a sequence located approximately 8-13 nucleotides upstream of the initiating AUG on mRNA, which can be precisely recognized by 16s rRNA through base complementarity [1]. This sequence promotes ribosome binding to mRNA, facilitating translation initiation. The length and base composition of the RBS sequence influence ribosome binding and translation efficiency to some extent [1]. A stronger RBS makes it easier for ribosomes to bind, thereby increasing gene expression levels, while a weaker RBS can reduce gene expression levels or only express under specific conditions.
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        Therefore, by replacing the RBS sequences that regulate the translation of the target protein, we can control the expression level of the target protein. We obtained a mutant of the original RBS (BBa_B0034) from the literature [2]. The RBS calculator showed a 108-fold decrease in the intensity of this mutant.
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    <h3>References</h3>
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    <p>[1] Kozak, M. Initiation of translation in prokaryotes and eukaryotes. Gene, 1999, 234(2), 187-208.</p>
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    <p>[2] Ji W, Shi H, Zhang H, Sun R, Xi J, Wen D, Feng J, Chen Y, Qin X, Ma Y, Luo W, Deng L, Lin H, Yu R, Ouyang Q. A formalized design process for bacterial consortia that perform logic computing. PLoS One. 2013;8(2): e57482.</p>
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Latest revision as of 14:38, 30 September 2024


B0034-mutant 2

RBS mutants with a 108-fold reduction in intensity over B0034.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


B0034-mutant 2 (RBS2) - BBa_K5348002

B0034-mutant 2 (RBS2) - BBa_K5348002

Profile

Name B0034-mutant 2 (RBS2)
Base Pairs 12 bp
Sequence GCTCCATCCCCG
Origin Synthesized

Properties

The ribosome binding site (RBS) is a sequence located approximately 8-13 nucleotides upstream of the initiating AUG on mRNA, which can be precisely recognized by 16s rRNA through base complementarity [1]. This sequence promotes ribosome binding to mRNA, facilitating translation initiation. The length and base composition of the RBS sequence influence ribosome binding and translation efficiency to some extent [1]. A stronger RBS makes it easier for ribosomes to bind, thereby increasing gene expression levels, while a weaker RBS can reduce gene expression levels or only express under specific conditions.

Therefore, by replacing the RBS sequences that regulate the translation of the target protein, we can control the expression level of the target protein. We obtained a mutant of the original RBS (BBa_B0034) from the literature [2]. The RBS calculator showed a 108-fold decrease in the intensity of this mutant.

References

[1] Kozak, M. Initiation of translation in prokaryotes and eukaryotes. Gene, 1999, 234(2), 187-208.

[2] Ji W, Shi H, Zhang H, Sun R, Xi J, Wen D, Feng J, Chen Y, Qin X, Ma Y, Luo W, Deng L, Lin H, Yu R, Ouyang Q. A formalized design process for bacterial consortia that perform logic computing. PLoS One. 2013;8(2): e57482.