Difference between revisions of "Part:BBa K243023:Design"

 
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<partinfo>BBa_K243023 short</partinfo>
 
<partinfo>BBa_K243023 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The linker length and the choice of the purification tag in combination with the anticalin tag allowed us several combination possiblities, which we had to prove to achieve the best combiantion.  
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The linker length and the choice of the purification tag in combination with the anticalin tag allowed us several combination possiblities, which we had to prove to achieve the best combiantion.
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[https://static.igem.org/mediawiki/parts/2/27/Freiburg09_Strep-FluA-LL-Fok_is.txt Commented GenBank file]
  
 
===Source===
 
===Source===

Revision as of 00:01, 22 October 2009

Strep-FluA-Long Linker-Fok_i


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The linker length and the choice of the purification tag in combination with the anticalin tag allowed us several combination possiblities, which we had to prove to achieve the best combiantion.

Commented GenBank file

Source

Combined by serial clonig steps.

References