Difference between revisions of "Part:BBa K187077"
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The biobytes assembly method can be used to rapidly assemble DNA segments in a particular order. The 3' end of constructs made with this method can be annealed to a terminator segment, which can be digested and annealed to the anchor to circularize the construct. | The biobytes assembly method can be used to rapidly assemble DNA segments in a particular order. The 3' end of constructs made with this method can be annealed to a terminator segment, which can be digested and annealed to the anchor to circularize the construct. | ||
| − | For more details see http://2009.igem.org/Team:Alberta/DNAanchor, or RFC 47 | + | For more details see http://2009.igem.org/Team:Alberta/DNAanchor, or RFC #47 |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 23:56, 21 October 2009
terminator reverse strand, Biobytes assembly method
This part can be annealed to the terminator forward strand A (BBa_K187067) or the terminator forward strand B (BBa_K18790), to produce an A or B overhang respectively. The A overhang can anneal to the A' end on the 3' end of a part produced from pBA. The B overhang can anneal to the B' overhang on the end of a part produced from pAB.
The biobytes assembly method can be used to rapidly assemble DNA segments in a particular order. The 3' end of constructs made with this method can be annealed to a terminator segment, which can be digested and annealed to the anchor to circularize the construct.
For more details see http://2009.igem.org/Team:Alberta/DNAanchor, or RFC #47
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]