Difference between revisions of "Part:BBa K5301008"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | We devised the fusion expression of mCherry[1-10] with spNW15, SpyCatcher and SdyCatcher (namely SCSdC-mCh[1-10]). | |
| + | We used mCherry[1-10] and mCherry[11] parts to emit fluorescence to verify the success of protien connection. If SCSdC-mCh[1-10] can connect to SnTST- mCh[11] successfully, the mCherry[1-10] and mCherry[11] parts can emit fluorescence as a characterization. | ||
===Characterization=== | ===Characterization=== | ||
Revision as of 13:36, 30 September 2024
MCherry [1-10] is a part of bimolecular fluorescence complementation (BiFC) system.
MCherry [1-10] is a part of bimolecular fluorescence complementation (BiFC) system which could visualize protein interactions. The combination of mCherry [1-10] and mCherry [11] can form the fluorescence of mCherry. With its advantages of a short maturation time and brilliant fluorescence, the mCherry BiFC system could find particular applications for analyzing protein–protein interactions especially in living cells [1].
Usage and Biology
We devised the fusion expression of mCherry[1-10] with spNW15, SpyCatcher and SdyCatcher (namely SCSdC-mCh[1-10]). We used mCherry[1-10] and mCherry[11] parts to emit fluorescence to verify the success of protien connection. If SCSdC-mCh[1-10] can connect to SnTST- mCh[11] successfully, the mCherry[1-10] and mCherry[11] parts can emit fluorescence as a characterization.
Characterization
We used SDS-PAGE to test whether
Fig.1
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 31
Illegal PstI site found at 230 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 31
Illegal PstI site found at 230 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 31
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 31
Illegal PstI site found at 230 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 31
Illegal PstI site found at 230 - 1000COMPATIBLE WITH RFC[1000]
- ↑ Fan, J., et al., Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein–protein interactions in living cells. Biochemical and Biophysical Research Communications, 2008. 367(1): p. 47-53.