Difference between revisions of "Part:BBa K5301011"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K5301011 short</partinfo>
 
<partinfo>BBa_K5301011 short</partinfo>
 +
 
Express the SpyCatcher-MSP1D1-SpyTag fusion protein to achieve self-cyclization of MSP1D1 for constructing nanodiscs, while the 6×His tag is used for subsequent protein purification.
 
Express the SpyCatcher-MSP1D1-SpyTag fusion protein to achieve self-cyclization of MSP1D1 for constructing nanodiscs, while the 6×His tag is used for subsequent protein purification.
  

Revision as of 13:14, 30 September 2024

spMSP1D1

Express the SpyCatcher-MSP1D1-SpyTag fusion protein to achieve self-cyclization of MSP1D1 for constructing nanodiscs, while the 6×His tag is used for subsequent protein purification.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 496
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 496
    Illegal NheI site found at 64
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 496
    Illegal BglII site found at 924
    Illegal BamHI site found at 97
    Illegal XhoI site found at 820
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 496
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 496
  • 1000
    COMPATIBLE WITH RFC[1000]