Difference between revisions of "Part:BBa K5301011"
Line 5: Line 5:
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
<partinfo>BBa K5301011 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K5301011 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa K5301011 parameters</partinfo>
+
<partinfo>BBa_K5301011 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 13:13, 30 September 2024

spMSP1D1 Express the SpyCatcher-MSP1D1-SpyTag fusion protein to achieve self-cyclization of MSP1D1 for constructing nanodiscs, while the 6×His tag is used for subsequent protein purification.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 496
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 496
    Illegal NheI site found at 64
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 496
    Illegal BglII site found at 924
    Illegal BamHI site found at 97
    Illegal XhoI site found at 820
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 496
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 496
  • 1000
    COMPATIBLE WITH RFC[1000]