Difference between revisions of "Part:BBa K5301028"

 
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<partinfo>BBa_K5301028 short</partinfo>
 
<partinfo>BBa_K5301028 short</partinfo>
  
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This pathway expresses the SpyCatcher-MSP1D1-SpyTag fusion protein under IPTG-induced conditions, achieving self-cyclization of MSP1D1 to construct nanodiscs.
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This pathway expresses the SpyCatcher-MSP1D1-SpyTag fusion protein (spMSP1D1) under IPTG-induced conditions, achieving self-cyclization of MSP1D1 to construct nanodiscs.For more information on spMSP1D1, go to <partinfo>BBa_K5301011</partinfo>.
  
 
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Revision as of 12:54, 30 September 2024


spMSP1D1 expression pathway (T7)

This pathway expresses the SpyCatcher-MSP1D1-SpyTag fusion protein (spMSP1D1) under IPTG-induced conditions, achieving self-cyclization of MSP1D1 to construct nanodiscs.For more information on spMSP1D1, go to BBa_K5301011.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 571
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 571
    Illegal NheI site found at 139
    Illegal NheI site found at 1145
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 571
    Illegal BglII site found at 999
    Illegal BamHI site found at 172
    Illegal XhoI site found at 895
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 571
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 571
  • 1000
    COMPATIBLE WITH RFC[1000]