Difference between revisions of "Part:BBa K5136043"

 
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<partinfo>BBa_K5136043 short</partinfo>
 
<partinfo>BBa_K5136043 short</partinfo>
  
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===Biology===
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pHybrid 2)-114 version is an engineering promoter that is suppressed by the Aca2 repressor, which uses the -35 and -10 regions of J23114.
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===Usage and design===
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We use pHybrid 2)-114 version as the promoter of the ccdA.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:45, 30 September 2024


pHybrid 2)-114 version

Biology

pHybrid 2)-114 version is an engineering promoter that is suppressed by the Aca2 repressor, which uses the -35 and -10 regions of J23114.

Usage and design

We use pHybrid 2)-114 version as the promoter of the ccdA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]