Difference between revisions of "Part:BBa K292008"
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This part contains a SV40 nucleus targeting sequence (DTS). This part is reliable to use to increase the level of gene transfection efficiency. <br>
 
This part contains a SV40 nucleus targeting sequence (DTS). This part is reliable to use to increase the level of gene transfection efficiency. <br>
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=== Usage and Biology ===
  
 
Any plasmid will rarely reach the nucleus if we do not provide to it, the necessary element to trigger a mechanism of nuclear incorporation. Studies were conducted on the use of specific sequences for nuclear import of genetic sequences: DTS sequences (DNA Nuclear Targeting Sequence). This bio-brick is able to play this role. <br>
 
Any plasmid will rarely reach the nucleus if we do not provide to it, the necessary element to trigger a mechanism of nuclear incorporation. Studies were conducted on the use of specific sequences for nuclear import of genetic sequences: DTS sequences (DNA Nuclear Targeting Sequence). This bio-brick is able to play this role. <br>

Revision as of 23:47, 21 October 2009

SV40 nucleus targeting sequence (DTS)


This part contains a SV40 nucleus targeting sequence (DTS). This part is reliable to use to increase the level of gene transfection efficiency.

Usage and Biology

Any plasmid will rarely reach the nucleus if we do not provide to it, the necessary element to trigger a mechanism of nuclear incorporation. Studies were conducted on the use of specific sequences for nuclear import of genetic sequences: DTS sequences (DNA Nuclear Targeting Sequence). This bio-brick is able to play this role.

Sequence:

5’ GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGAAGGTACC T 3’

This sequence, known for having the ability to bind to 10 different transcription factors, provides the transportation of DNA to the nucleus. Added to the therapeutic plasmid, this sequence ensures the entry and the expression of therapeutic plasmid into the nucleus of the target cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


biology-NA-
direction-NA-