Difference between revisions of "Part:BBa K5097008"
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− | The 2024 Oneonta iGEM team pHish and CHIPs created this acid-sensitive reporter circuit to characterize the expression of genes in response to acidic conditions, using | + | ===Usage and Biology=== |
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+ | The 2024 Oneonta iGEM team pHish and CHIPs created this acid-sensitive reporter circuit to characterize the expression of genes in response to acidic conditions, using pASR (BBa_K1231000). To do this, we used pARS to drive the expression of RFP, a reporter gene. | ||
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Revision as of 12:25, 30 September 2024
pASR-RFP reporter
Usage and Biology
The 2024 Oneonta iGEM team pHish and CHIPs created this acid-sensitive reporter circuit to characterize the expression of genes in response to acidic conditions, using pASR (BBa_K1231000). To do this, we used pARS to drive the expression of RFP, a reporter gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 707
Illegal AgeI site found at 819 - 1000COMPATIBLE WITH RFC[1000]