Difference between revisions of "Part:BBa K5184015"

 
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In order to manufacture zingiberene from glucose, we incorporate SltNPPS (part number), an neryl diphosphate synthase with its N-termial 45 amino acid-trucated. Originally found in Solanum Lycopersium, it is used for transformation of glucose into IPP and DMAPP.                                                            Mvan4662 (part number), a farnesyl diphosphate synthase facilitating the formation of longer-chain isoprenoid diphosphates. In our context, it is included for synthesis of Z,Z-FPP from NPP.                        Lastly, ShZIS, a zingiberene synthase derived from Solanum habrochaites, catalyzes the formation of sesquiterpenes, a compound with 15 carbons. Specifically, we us ShZIS to produce 7-epi-zingiberene from its substrate Z,Z-FPP.                                                                                                                Through incorporation of the three enzymes, we can obtain our desired product from glucose in a straightforward pathway.
 
In order to manufacture zingiberene from glucose, we incorporate SltNPPS (part number), an neryl diphosphate synthase with its N-termial 45 amino acid-trucated. Originally found in Solanum Lycopersium, it is used for transformation of glucose into IPP and DMAPP.                                                            Mvan4662 (part number), a farnesyl diphosphate synthase facilitating the formation of longer-chain isoprenoid diphosphates. In our context, it is included for synthesis of Z,Z-FPP from NPP.                        Lastly, ShZIS, a zingiberene synthase derived from Solanum habrochaites, catalyzes the formation of sesquiterpenes, a compound with 15 carbons. Specifically, we us ShZIS to produce 7-epi-zingiberene from its substrate Z,Z-FPP.                                                                                                                Through incorporation of the three enzymes, we can obtain our desired product from glucose in a straightforward pathway.
  
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===Abstract===
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===Usage and Biology===
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SltNPPS-Mvan4662-ShZIS is a pathway of enzymes used for the synthesis of 7epiZ in E. coli. SltNPPS transforms IPP and DMAPP from the MVA pathway to NPP. Mvan4662 catalyzes the formation of Z,Z-FPP from NPP. Lastly, ShZIS produces 7epiZ from Z,Z-FPP.
  
 
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<partinfo>BBa_K5184015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5184015 SequenceAndFeatures</partinfo>
  
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===Usage and Biology===
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SltNPPS is a cis-prenyl transferase from Solanum Lycopersium with its N-terminal 45 amino acid truncated that catalyzes the conversion of geranyl diphosphate into neryl diphosphate. Mvan4662 is a cis-farnesyl diphosphate synthase that catalyzes the transfer of an isopentenyl group from isopentenyl pyrophosphate (IPP) to prenyl diphosphates, facilitating the formation of longer-chain isoprenoid diphosphates. ShZIS is a sesquiterpene synthase derived from Solanum habrochaites, and is present and expressed in the tomato's glandular trichomes. By utilizing three isoprenoid units, ShZIS catalyzes the formation of sesquiterpenes, a compound with 15 carbons. Intermediates that are commonly used include isopentenyl diphosphate (IPP), dimethylallyl diphosphate (DMAPP), and (E,E)-α-farnesyl diphosphate (FPP).
  
 
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Revision as of 12:21, 30 September 2024


SltNPPS-Mvan4662-ShZIS

In order to manufacture zingiberene from glucose, we incorporate SltNPPS (part number), an neryl diphosphate synthase with its N-termial 45 amino acid-trucated. Originally found in Solanum Lycopersium, it is used for transformation of glucose into IPP and DMAPP. Mvan4662 (part number), a farnesyl diphosphate synthase facilitating the formation of longer-chain isoprenoid diphosphates. In our context, it is included for synthesis of Z,Z-FPP from NPP. Lastly, ShZIS, a zingiberene synthase derived from Solanum habrochaites, catalyzes the formation of sesquiterpenes, a compound with 15 carbons. Specifically, we us ShZIS to produce 7-epi-zingiberene from its substrate Z,Z-FPP. Through incorporation of the three enzymes, we can obtain our desired product from glucose in a straightforward pathway.

Abstract

SltNPPS-Mvan4662-ShZIS is a pathway of enzymes used for the synthesis of 7epiZ in E. coli. SltNPPS transforms IPP and DMAPP from the MVA pathway to NPP. Mvan4662 catalyzes the formation of Z,Z-FPP from NPP. Lastly, ShZIS produces 7epiZ from Z,Z-FPP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

SltNPPS is a cis-prenyl transferase from Solanum Lycopersium with its N-terminal 45 amino acid truncated that catalyzes the conversion of geranyl diphosphate into neryl diphosphate. Mvan4662 is a cis-farnesyl diphosphate synthase that catalyzes the transfer of an isopentenyl group from isopentenyl pyrophosphate (IPP) to prenyl diphosphates, facilitating the formation of longer-chain isoprenoid diphosphates. ShZIS is a sesquiterpene synthase derived from Solanum habrochaites, and is present and expressed in the tomato's glandular trichomes. By utilizing three isoprenoid units, ShZIS catalyzes the formation of sesquiterpenes, a compound with 15 carbons. Intermediates that are commonly used include isopentenyl diphosphate (IPP), dimethylallyl diphosphate (DMAPP), and (E,E)-α-farnesyl diphosphate (FPP).