Difference between revisions of "Part:BBa K5097015"
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<partinfo>BBa_K5097015 short</partinfo> | <partinfo>BBa_K5097015 short</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
This part is a derivative of part BBa_E1010 which codes for a highly engineered mutant of red fluorescent protein from Discosoma striata (coral), added to the parts repository in 2004. This iteration of RFP contains the same coding sequence, but has had the double stop codon and the DNA barcode removed. Removal of these sequences allows for the addition of a C-terminal tag, or to create fusion proteins with RFP. | This part is a derivative of part BBa_E1010 which codes for a highly engineered mutant of red fluorescent protein from Discosoma striata (coral), added to the parts repository in 2004. This iteration of RFP contains the same coding sequence, but has had the double stop codon and the DNA barcode removed. Removal of these sequences allows for the addition of a C-terminal tag, or to create fusion proteins with RFP. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 11:56, 30 September 2024
RFP without a stop codon
Usage and Biology
This part is a derivative of part BBa_E1010 which codes for a highly engineered mutant of red fluorescent protein from Discosoma striata (coral), added to the parts repository in 2004. This iteration of RFP contains the same coding sequence, but has had the double stop codon and the DNA barcode removed. Removal of these sequences allows for the addition of a C-terminal tag, or to create fusion proteins with RFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000COMPATIBLE WITH RFC[1000]