Difference between revisions of "Part:BBa K5226043:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Although this part complies with one of the RFC 10 or Type IIS standards, it is inconsistent with the standards upheld by other basic parts. As a result, the composite component formed by their combination has illegal sites, necessitating a synonymous mutation.The base pairs at 183 bp and 555 bp were changed from a/t to g/c, and the base pairs at 444 bp were changed from g/c to a/t. | + | Although this part complies with one of the RFC 10 or Type IIS standards, it is inconsistent with the standards upheld by other basic parts. As a result, the composite component formed by their combination has illegal sites, necessitating a synonymous mutation.The base pairs at 183 bp and 555 bp were changed from a/t to g/c, and the base pairs at 444 bp and 345 bp were changed from g/c to a/t. |
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 11:32, 30 September 2024
stth
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1084
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 213
Illegal NgoMIV site found at 471
Illegal NgoMIV site found at 1174
Illegal NgoMIV site found at 1440
Illegal AgeI site found at 359
Illegal AgeI site found at 700 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Although this part complies with one of the RFC 10 or Type IIS standards, it is inconsistent with the standards upheld by other basic parts. As a result, the composite component formed by their combination has illegal sites, necessitating a synonymous mutation.The base pairs at 183 bp and 555 bp were changed from a/t to g/c, and the base pairs at 444 bp and 345 bp were changed from g/c to a/t.
Source
Synthetic（BGI）. Sequence source is from Streptomyces toxytricini, found through NCBI （https://www.ncbi.nlm.nih.gov/protein/E9P162.1）