Difference between revisions of "Part:BBa K5460000"

 
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        <p><strong>Reporting assay with Standardized Interfaces</strong><br>This part serves as a reporting module (R module) of our system. In some cases, this plasmid needs to be used in conjunction with our A module. To align with our hardware system for simultaneous multi-biomarker detection, we require various biomarker-sensitive promoters and different fluorescent proteins to avoid signal cross-talk.<br>We optimized and improved the plasmid system by introducing a GoldenGate interface at key sites within this module. Specifically, we designed a standardized GoldenGate interface at the positions of the promoter and the fluorescent protein.</p>
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            <img src="https://static.igem.wiki/teams/5460/part-registry/r.jpg" alt="图一" style="width: 600px; margin-right: 10px;">
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        <p>This allows us to easily integrate different promoters into the plasmid system through a simple GoldenGate reaction. Using distinct fluorescent signal channels to report different biomarkers, this system provides the biotechnological foundation for our hardware. <br>Through this reporting system, we tested the response curves of four different biomarkers—uric acid, glucose, tryptophan, and lactate—using three fluorescent proteins: mKate2, sfGFP, and mTagBF2. The data were fitted to Hill equations for analysis:</p>
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    <img src="https://static.igem.wiki/teams/5460/part-registry/glu-curve.jpg" alt="图2" style="width: 225px; margin-right: 10px;">
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            <img src="https://static.igem.wiki/teams/5460/part-registry/latic-curve.jpg" alt="图3" style="width: 225px; margin-right: 10px;">
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            <img src="https://static.igem.wiki/teams/5460/part-registry/trp-curve.jpg" alt="图4" style="width: 225px;">
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        <img src="https://static.igem.wiki/teams/5460/part-registry/ua-curve.jpg" alt="图4" style="width: 225px;">
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      <p>In the future, this system can be adapted to detect other biomarkers by replacing the plasmid accordingly. Additionally, we have developed a hardware system designed to work in conjunction with this detection system. For more details, please refer to our <strong>Hardware</strong>page.</p>
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Revision as of 11:07, 30 September 2024

Reporting assay with Standardized Interfaces
This part serves as a reporting module (R module) of our system. In some cases, this plasmid needs to be used in conjunction with our A module. To align with our hardware system for simultaneous multi-biomarker detection, we require various biomarker-sensitive promoters and different fluorescent proteins to avoid signal cross-talk.
We optimized and improved the plasmid system by introducing a GoldenGate interface at key sites within this module. Specifically, we designed a standardized GoldenGate interface at the positions of the promoter and the fluorescent protein.

图一

This allows us to easily integrate different promoters into the plasmid system through a simple GoldenGate reaction. Using distinct fluorescent signal channels to report different biomarkers, this system provides the biotechnological foundation for our hardware.
Through this reporting system, we tested the response curves of four different biomarkers—uric acid, glucose, tryptophan, and lactate—using three fluorescent proteins: mKate2, sfGFP, and mTagBF2. The data were fitted to Hill equations for analysis:

图2 图3 图4 图4

In the future, this system can be adapted to detect other biomarkers by replacing the plasmid accordingly. Additionally, we have developed a hardware system designed to work in conjunction with this detection system. For more details, please refer to our Hardwarepage.